EC |
1.14.14.183 |
Accepted name: |
taxoid 2α-hydroxylase |
Reaction: |
(1) taxusin + [reduced NADPH—hemoprotein reductase] + O2 = 2α-hydroxytaxusin + [oxidized NADPH—hemoprotein reductase] + H2O (2) 7β-hydroxytaxusin + [reduced NADPH—hemoprotein reductase] + O2 = 2α,7β-dihydroxytaxusin + [oxidized NADPH—hemoprotein reductase] + H2O |
Glossary: |
taxusin = taxa-4(20),11-diene-5α,9α,10β,13α-tetrayl tetraacetate |
Systematic name: |
taxusin, [reduced NADPH—hemoprotein reductase]:oxygen 2-oxidoreductase |
Comments: |
A cytochrome P-450 (heme-thiolate) protein from the yew tree Taxus cuspidata. Does not act on earlier intermediates in taxol biosynthesis. |
References: |
1. |
Chau, M., Jennewein, S., Walker, K. and Croteau, R. Taxol biosynthesis: Molecular cloning and characterization of a cytochrome P450 taxoid 7β-hydroxylase. Chem. Biol. 11 (2004) 663–672. [PMID: 15157877] |
2. |
Chau, M. and Croteau, R. Molecular cloning and characterization of a cytochrome P450 taxoid 2α-hydroxylase involved in taxol biosynthesis. Arch. Biochem. Biophys. 427 (2004) 48–57. [PMID: 15178487] |
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[EC 1.14.14.183 created 2022] |
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