ExplorEnz: EC 2.4.1.395

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2.4.1.395

Accepted name:

reuteransucrase

Reaction:

formation of reuteran from sucrose

Glossary:

reuteran = a high-molecular-mass branched α-glucan produced by the lactic acid bacterium Limosilactobacillus reuteri.

Systematic name:

sucrose:α-D-glucan 4-α/6-α-D-glucosyltransferase

Comments:

The glucansucrases transfer a D-glucosyl residue from sucrose to a glucan chain. They are classified based on the linkage of the transferred glucosyl residue. The enzyme, characterized from the lactic acid bacterium Limosilactobacillus reuteri strain 121, catalyses the hydrolysis of sucrose and the transfer of the D-glucose moiety to suitable acceptors (inclduing sucrose), forming the glucan reuteran, which is typical for these strains. The enzyme forms mostly α(1→4) glucosidic linkages, but also α(1→6) linkages. The presence of maltose significantly accelerate the initial rate of the reaction. See EC 2.4.1.5, dextransucrase.

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc

References:

1.	Kralj, S., van Geel-Schutten, G.H., Rahaoui, H., Leer, R.J., Faber, E.J., van der Maarel, M.J. and Dijkhuizen, L. Molecular characterization of a novel glucosyltransferase from Lactobacillus reuteri strain 121 synthesizing a unique, highly branched glucan with α-(1→4) and α-(1→6) glucosidic bonds. Appl. Environ. Microbiol. 68 (2002) 4283–4291. [DOI] [PMID: 12200277]
2.	Kralj, S., van Geel-Schutten, G.H., van der Maarel, M.JE.C. and Dijkhuizen, L. Biochemical and molecular characterization of Lactobacillus reuteri 121 reuteransucrase. Microbiology (Reading) 150 (2004) 2099–2112. [DOI] [PMID: 15256553]
3.	Kralj, S., Stripling, E., Sanders, P., van Geel-Schutten, G.H. and Dijkhuizen, L. Highly hydrolytic reuteransucrase from probiotic Lactobacillus reuteri strain ATCC 55730. Appl. Environ. Microbiol. 71 (2005) 3942–3950. [DOI] [PMID: 16000808]

[EC 2.4.1.395 created 2023]