ExplorEnz: EC 3.1.1.122

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3.1.1.122

Accepted name:

carbendazim hydrolysing esterase

Reaction:

carbendazim + H₂O = 2-aminobenzimidazole + CO₂ + methanol (overall reaction)
(1a) carbendazim + H₂O = N-(1H-1,3-benzodiazol-2-yl)carbamate + methanol
(1b) N-(1H-1,3-benzodiazol-2-yl)carbamate = 2-aminobenzimidazole + CO₂ (spontaneous)

Glossary:

carbendazim = methyl 1H-benzimidazol-2-ylcarbamate; 2-aminobenzimidazole = 1H-benzimidazol-2-amine

Other name(s):

mheI (gene name)

Systematic name:

carbendazim methanol hydrolase (decarboxylating)

Comments:

The enzyme, which is inducible in the soil bacterium Nocardioides sp. (strain SG-4G), catalyses the degradation of the fungicide carbendazim. Following hydrolysis of the carbamate ester, the carbamate decarboxylates spontaneously.

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 10605-21-7

References:

Pandey, G., Dorrian, S.J., Russell, R.J., Brearley, C., Kotsonis, S. and Oakeshott, J.G. Cloning and biochemical characterization of a novel carbendazim (methyl-1H-benzimidazol-2-ylcarbamate)-hydrolyzing esterase from the newly isolated Nocardioides sp. strain SG-4G and its potential for use in enzymatic bioremediation. Appl. Environ. Microbiol. 76 (2010) 2940–2945. [DOI] [PMID: 20228105]

[EC 3.1.1.122 created 2023]