ExplorEnz: EC 3.2.1.223

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3.2.1.223

Accepted name:

arabinogalactan exo α-(1,3)-β-L-arabinopyranosyl-(1→3)-L-arabinofuranosidase (non-reducing end)

Reaction:

Hydrolysis of β-L-Arap-(1→3)-L-Araf disaccharides from non-reducing terminals in branches of type II arabinogalactan attached to proteins.

Glossary:

Araf = arabinofuranose
Arap = arabinopyranose

Other name(s):

3-O-β-L-arabinopyranosyl-α-L-arabinofuranosidase; AAfase

Systematic name:

type II arabinogalactan exo α-(1,3)-[β-L-arabinopyranosyl-(1→3)-L-arabinofuranose] hydrolase (non-reducing end)

Comments:

The enzyme, characterized from the bacterium Bifidobacterium pseudocatenulatum, specifically hydrolyses β;-L-Arap-(1→3)-L-Araf disaccharides from the non-reducing terminal of arabinogalactan using an exo mode of action. It is active with arabinogalactan-proteins (AGPs) containing type II arabinogalactans such as gum arabic AGP and larch AGP. The enzyme can also hydrolyse α-D-Galp-(1→3)-L-Araf disaccharides (cf. EC 3.2.1.215) with a much lower activity.

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc

References:

1.	Sasaki, Y., Yanagita, M., Hashiguchi, M., Horigome, A., Xiao, J. Z., Odamaki, T., Kitahara, K. and Fujita, K. Assimilation of arabinogalactan side chains with novel 3-O-β-L-arabinopyranosyl-α-L-arabinofuranosidase in Bifidobacterium pseudocatenulatum. Microbiome Res. Rep. 2:12 (2023). [DOI]

[EC 3.2.1.223 created 2023]