EC |
1.14.13.228 |
Accepted name: |
jasmonic acid 12-hydroxylase |
Reaction: |
(–)-jasmonate + NADPH + H+ + O2 = trans-12-hydroxyjasmonate + NADP+ + H2O |
Glossary: |
(–)-jasmonate = {(1R,2R)-3-oxo-2-[(2Z)-pent-2-en-1-yl]cyclopentyl}acetate
trans-12-hydroxyjasmonate = {(1R,2R)-2-[(2Z)-5-hydroxypent-2-en-1-yl]-3-oxocyclopentyl}acetate |
Other name(s): |
ABM (gene name) |
Systematic name: |
jasmonate,NADPH:oxygen oxidoreductase (12-hydroxylating) |
Comments: |
Although believed to occur in plants, the enzyme has so far been characterized only from the rice blast fungus, Magnaporthe oryzae. The fungus strategically deploys the enzyme to hydroxylate and inactivate endogenous jasmonate to evade the jasmonate-based innate immunity in rice plants. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc |
References: |
1. |
Patkar, R.N., Benke, P.I., Qu, Z., Chen, Y.Y., Yang, F., Swarup, S. and Naqvi, N.I. A fungal monooxygenase-derived jasmonate attenuates host innate immunity. Nat. Chem. Biol. 11 (2015) 733–740. [DOI] [PMID: 26258762] |
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[EC 1.14.13.228 created 2016] |
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EC |
2.8.2.39 |
Accepted name: |
hydroxyjasmonate sulfotransferase |
Reaction: |
3′-phosphoadenylyl-sulfate + 12-hydroxyjasmonate = adenosine 3′,5′-bisphosphate + 12-sulfooxyjasmonate |
Glossary: |
12-hydroxyjasmonate = {(1R,2R)-2-[(2E)-5-hydroxypent-2-enyl]-3-oxocyclopentyl}acetate |
Other name(s): |
ST2A (gene name); 3′-phosphoadenylyl-sulfate:12-hydroxyjasmonate sulfotransferase |
Systematic name: |
3′-phosphoadenylyl-sulfate:12-hydroxyjasmonate sulfonotransferase |
Comments: |
The enzyme, charaterized from the plant Arabidopsis thaliana, also acts on 11-hydroxyjasmonate. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc |
References: |
1. |
Gidda, S.K., Miersch, O., Levitin, A., Schmidt, J., Wasternack, C. and Varin, L. Biochemical and molecular characterization of a hydroxyjasmonate sulfotransferase from Arabidopsis thaliana. J. Biol. Chem. 278 (2003) 17895–17900. [DOI] [PMID: 12637544] |
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[EC 2.8.2.39 created 2017] |
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EC |
3.5.1.127 |
Accepted name: |
jasmonoyl-L-amino acid hydrolase |
Reaction: |
a jasmonoyl-L-amino acid + H2O = jasmonate + an L-amino acid |
Glossary: |
tuberonic acid = 12-hydroxyjasmonate = {(1R,2R)-2-[(2Z)-5-hydroxypent-2-enyl]-3-oxo-cyclopentyl}acetate
jasmonate = {(1R,2R)-3-oxo-2-[(2Z)-pent-2-enyl]cyclopentyl}acetate |
Other name(s): |
IAR3 (gene name); ILL4 (gene name); ILL6 (gene name) |
Systematic name: |
jasmonoyl-L-amino acid amidohydrolase |
Comments: |
This entry includes a family of enzymes that recyle jasmonoyl-amino acid conjugates back to jasmonates. The enzymes from Arabidopsis thaliana have been shown to also act on 12-hydroxyjasmonoyl-L-isoleucine, generating tuberonic acid. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc |
References: |
1. |
Widemann, E., Miesch, L., Lugan, R., Holder, E., Heinrich, C., Aubert, Y., Miesch, M., Pinot, F. and Heitz, T. The amidohydrolases IAR3 and ILL6 contribute to jasmonoyl-isoleucine hormone turnover and generate 12-hydroxyjasmonic acid upon wounding in Arabidopsis leaves. J. Biol. Chem. 288 (2013) 31701–31714. [DOI] [PMID: 24052260] |
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[EC 3.5.1.127 created 2017] |
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