EC |
1.3.8.12 |
Accepted name: |
(2S)-methylsuccinyl-CoA dehydrogenase |
Reaction: |
(2S)-methylsuccinyl-CoA + electron-transfer flavoprotein = 2-methylfumaryl-CoA + reduced electron-transfer flavoprotein |
Glossary: |
2-methylfumaryl-CoA = (E)-3-carboxy-2-methylprop-2-enoyl-CoA |
Other name(s): |
Mcd |
Systematic name: |
(2S)-methylsuccinyl-CoA:electron-transfer flavoprotein oxidoreductase |
Comments: |
The enzyme, characterized from the bacterium Rhodobacter sphaeroides, is involved in the ethylmalonyl-CoA pathway for acetyl-CoA assimilation. The enzyme contains FAD. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc |
References: |
1. |
Erb, T.J., Fuchs, G. and Alber, B.E. (2S)-Methylsuccinyl-CoA dehydrogenase closes the ethylmalonyl-CoA pathway for acetyl-CoA assimilation. Mol. Microbiol. 73 (2009) 992–1008. [DOI] [PMID: 19703103] |
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[EC 1.3.8.12 created 2015] |
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EC |
2.8.3.26 |
Accepted name: |
succinyl-CoA:mesaconate CoA transferase |
Reaction: |
succinyl-CoA + mesaconate = 2-methylfumaryl-CoA + succinate |
Glossary: |
2-methylfumaryl-CoA = (E)-3-carboxy-2-methylprop-2-enoyl-CoA
mesaconate = 2-methylbut-2-enedioic acid |
Other name(s): |
mct (gene name) |
Systematic name: |
succinyl-CoA:mesaconate CoA transferase |
Comments: |
The enzyme participates in the methylaspartate cycle, an anaplerotic pathway that operates in some members of the haloarchaea and forms malate from acetyl-CoA. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc |
References: |
1. |
Khomyakova, M., Bukmez, O., Thomas, L.K., Erb, T.J. and Berg, I.A. A methylaspartate cycle in haloarchaea. Science 331 (2011) 334–337. [PMID: 21252347] |
2. |
Borjian, F., Johnsen, U., Schonheit, P. and Berg, I.A. Succinyl-CoA:mesaconate CoA-transferase and mesaconyl-CoA hydratase, enzymes of the methylaspartate cycle in Haloarcula hispanica. Front. Microbiol. 8:1683 (2017). [PMID: 28932214] |
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[EC 2.8.3.26 created 2020] |
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EC |
4.2.1.148 |
Accepted name: |
2-methylfumaryl-CoA hydratase |
Reaction: |
(2R,3S)-2-methylmalyl-CoA = 2-methylfumaryl-CoA + H2O |
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For diagram of the 3-hydroxypropanoate cycle, click here |
Glossary: |
(2R,3S)-2-methylmalyl-CoA = L-erythro-β-methylmalyl-CoA = (2R,3S)-2-methyl-3-carboxy-3-hydroxypropanoyl-CoA
2-methylfumaryl-CoA = (E)-3-carboxy-2-methylprop-2-enoyl-CoA
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Other name(s): |
Mcd; erythro-β-methylmalonyl-CoA hydrolyase; mesaconyl-coenzyme A hydratase (ambiguous); mesaconyl-C1-CoA hydratase |
Systematic name: |
(2R,3S)-2-methylmalyl-CoA hydro-lyase (2-methylfumaryl-CoA-forming) |
Comments: |
The enzyme from the bacterium Chloroflexus aurantiacus is part of the 3-hydroxypropanoate cycle for carbon assimilation. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB |
References: |
1. |
Zarzycki, J., Schlichting, A., Strychalsky, N., Muller, M., Alber, B.E. and Fuchs, G. Mesaconyl-coenzyme A hydratase, a new enzyme of two central carbon metabolic pathways in bacteria. J. Bacteriol. 190 (2008) 1366–1374. [DOI] [PMID: 18065535] |
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[EC 4.2.1.148 created 2014] |
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EC |
5.4.1.3 |
Accepted name: |
2-methylfumaryl-CoA isomerase |
Reaction: |
2-methylfumaryl-CoA = 3-methylfumaryl-CoA |
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For diagram of the 3-hydroxypropanoate cycle, click here |
Glossary: |
2-methylfumaryl-CoA = (E)-3-carboxy-2-methylprop-2-enoyl-CoA
3-methylfumaryl-CoA = (E)-3-carboxybut-2-enoyl-CoA |
Other name(s): |
mesaconyl-CoA C1-C4 CoA transferase; Mct |
Systematic name: |
2-methylfumaryl-CoA 1,4-CoA-mutase |
Comments: |
The enzyme, purified from the bacterium Chloroflexus aurantiacus, acts as an intramolecular CoA transferase and does not transfer CoA to free mesaconate. It is part of the 3-hydroxypropanoate cycle for carbon assimilation. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB |
References: |
1. |
Zarzycki, J., Brecht, V., Muller, M. and Fuchs, G. Identifying the missing steps of the autotrophic 3-hydroxypropionate CO2 fixation cycle in Chloroflexus aurantiacus. Proc. Natl. Acad. Sci. USA 106 (2009) 21317–21322. [DOI] [PMID: 19955419] |
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[EC 5.4.1.3 created 2014] |
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