EC |
2.4.1.211 |
Accepted name: |
1,3-β-galactosyl-N-acetylhexosamine phosphorylase |
Reaction: |
β-D-galactopyranosyl-(1→3)-N-acetyl-D-glucosamine + phosphate = α-D-galactopyranose 1-phosphate + N-acetyl-D-glucosamine |
Other name(s): |
lacto-N-biose phosphorylase; LNBP; galacto-N-biose phosphorylase |
Systematic name: |
β-D-galactopyranosyl-(1→3)-N-acetyl-D-hexosamine:phosphate galactosyltransferase |
Comments: |
Reaction also occurs with β-D-galactopyranosyl-(1→3)-N-acetyl-D-galactosamine as the substrate, giving N-acetyl-D-galactosamine as the product. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 224427-06-9 |
References: |
1. |
Derensy-Dron, D., Krzewinski, F., Brassart, C. and Bouquelet S. β-1,3-Galactosyl-N-acetylhexosamine phosphorylase from Bifidobacterium bifidum DSM 20082: characterization, partial purification and relation to mucin degradation. Biotechnol. Appl. Biochem. 29 (1999) 3–10. [PMID: 9889079] |
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[EC 2.4.1.211 created 2001] |
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EC |
2.4.1.341 |
Accepted name: |
α-1,2-colitosyltransferase |
Reaction: |
GDP-β-L-colitose + β-D-galactopyranosyl-(1→3)-N-acetyl-D-glucosamine = GDP + α-L-colitosyl-(1→2)-β-D-galactosyl-(1→3)-N-acetyl-D-glucosamine |
Glossary: |
β-D-galactopyranosyl-(1→3)-N-acetyl-D-glucosamine = lacto-N-biose |
Other name(s): |
wbgN (gene name) |
Systematic name: |
GDP-β-L-colitose:β-D-galactopyranosyl-(1→3)-N-acetyl-D-glucosamine L-colitosyltransferase (configuration-inverting) |
Comments: |
The enzyme, characterized from the bacterium Escherichia coli O55:H7, participates in the biosynthesis of an O-antigen. The reaction involves anomeric inversion, and does not require any metal ions. The enzyme is highly specific towards the acceptor, exclusively recognizing lacto-N-biose, but can accept GDP-L-fucose as the donor with almost the same activity as with GDP-β-L-colitose. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc |
References: |
1. |
Wu, Z., Zhao, G., Li, T., Qu, J., Guan, W., Wang, J., Ma, C., Li, X., Zhao, W., Wang, P.G. and Li, L. Biochemical characterization of an α1,2-colitosyltransferase from Escherichia coli O55:H7. Glycobiology (2015) . [DOI] [PMID: 26703456] |
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[EC 2.4.1.341 created 2016] |
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