EC |
1.3.8.15 |
Accepted name: |
3-(aryl)acrylate reductase |
Reaction: |
(1) phloretate + electron-transfer flavoprotein = 4-coumarate + reduced electron-transfer flavoprotein (2) 3-phenylpropanoate + electron-transfer flavoprotein = trans-cinnamate + reduced electron-transfer flavoprotein (3) 3-(1H-indol-3-yl)propanoate + electron-transfer flavoprotein = 3-(indol-3-yl)acrylate + reduced electron-transfer flavoprotein
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Glossary: |
phloretate = 3-(4-hydroxyphenyl)propanoate
crotonate = (2E)-but-2-enoate |
Other name(s): |
acdA (gene name) |
Systematic name: |
3-(phenyl)propanoate:electron-transfer flavoprotein 2,3-oxidoreductase |
Comments: |
The enzyme, found in some amino acid-fermenting anaerobic bacteria, participates in the fermentation pathways of L-phenylalanine, L-tyrosine, and L-tryptophan. Unlike EC 1.3.1.31, 2-enoate reductase, this enzyme has minimal activity with crotonate. |
References: |
1. |
Dodd, D., Spitzer, M.H., Van Treuren, W., Merrill, B.D., Hryckowian, A.J., Higginbottom, S.K., Le, A., Cowan, T.M., Nolan, G.P., Fischbach, M.A. and Sonnenburg, J.L. A gut bacterial pathway metabolizes aromatic amino acids into nine circulating metabolites. Nature 551 (2017) 648–652. [PMID: 29168502] |
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[EC 1.3.8.15 created 2019] |
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EC |
3.7.1.4 |
Accepted name: |
phloretin hydrolase |
Reaction: |
phloretin + H2O = phloretate + phloroglucinol |
Glossary: |
phloretin = 3-(4-hydroxyphenyl)-1-(2,4,6-trihydroxyphenyl)propan-1-one
phloretate = 3-(4-hydroxyphenyl)propanoate
phloroglucinol = benzene-1,3,5-triol |
Other name(s): |
ErPhy; lactase-phlorerin hydrolase; C-acylphenol hydrolase; 2′,4,4′,6′-tetrahydroxydehydrochalcone 1,3,5-trihydroxybenzenehydrolase (incorrect) |
Systematic name: |
phloretin acylhydrolase (phloroglucinol-forming) |
Comments: |
Also hydrolyses other C-acylated phenols related to phloretin. Isolated from the fungus Aspergillus niger and the bacteria Pantoea agglomerans and Eubacterium ramulus. |
References: |
1. |
Chatterjee, A.K. and Gibbins, L.N. Metabolism of phloridzin by Erwinia herbicola: nature of the degradation products, and the purification and properties of phloretin hydrolase. J. Bacteriol. 100 (1969) 594–600. [PMID: 5354935] |
2. |
Minamikawa, T., Jayasankar, N.P., Bohm, B.A., Taylor, I.E. and Towers, G.H. An inducible hydrolase from Aspergillus niger, acting on carbon-carbon bonds, for phlorrhizin and other C-acylated phenols. Biochem. J. 116 (1970) 889–897. [PMID: 5441377] |
3. |
Schoefer, L., Braune, A. and Blaut, M. Cloning and expression of a phloretin hydrolase gene from Eubacterium ramulus and characterization of the recombinant enzyme. Appl. Environ. Microbiol. 70 (2004) 6131–6137. [PMID: 15466559] |
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[EC 3.7.1.4 created 1972, modified 2018] |
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